A villainess who suffers a notable embarrassment which undermines her dignity/arrogance - she may be made to seem ridiculous/pathetic/totally outclassed.The context may be comic, though not invariably. Often humiliation would occur in the course of a villainesses defeat/capture. Examples of this can include an adult villainess who is defeated/captured by a child; a physically tall/large villainess defeated/captured by a much smaller adult. Acts causing physical humiliation, can include things like being splattered with mud/paint etc, or receiving a kick in the backside.
There is a separate category to be used when the humiliation alone is the main fate of the villainess.